Urease Catalysis

Urease Catalysis. Ii. Inhibition of the Enzyme by Hydroxyurea, Hydroxylamine, and Acetohydroxamic Acid.

In the preceding paper (l), hydroxyurea was shown to be a substrate for urease and to be associated with the development of a marked inhibition of the enzyme during t,he course of the reaction. The lack of significant inhibition during the hydrolysis of urea at equal concentrations suggested that the hydroxylamine formed during hydroxyurea hydrolysis was the responsible agent. The inhibitory ef...

Gastrointestinal urease in man ' Part I Activity of mucosal urease

The relationship postulated between hepatic coma and hyperammonaemia implicates the gastrointestinal tract as a major source of the excess ammonia in the peripheral blood of some patients with cirrhosis (Butt and Summerskill, 1961; Chalmers, 1960; Sherlock, Summerskill, White, and Phear, 1954). Conventional therapy, comprising protein restriction, antibiotics, and enemas, is directed toward eli...

Studies on Crystalline Urease

1. Crystalline urease is not inactivated by trypsin in the absence of a gum. In fact, the presence of trypsin alone in aqueous solutions of urease has an action similar to that of gum, that is, it acts as a "protective colloid" for urease. 2. Crystalline urease is inactivated by trypsin in the presence of a gum. This occurs with great rapidity if purified (crystalline) trypsin is used. 3. If tr...

Homogeneous Catalysis Homogeneous Catalysis

Urease inhibitors: A review

Urease inhibitors possess a wide application in agriculture, clinical science and understanding of enzyme kinetics. These inhibitors play a significant role in reducing the loss of nitrogen from urea fertilizer in the form of volatile ammonia and, thus, improve the utility of urea based fertilizers. Their impact as an effective drug against urease producing bacterial infection is well documente...